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Synthesis, characterization, DNA binding and cytotoxicity studies of moxifloxacinato complexes

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Abstract

Five metal complexes of the third-generation quinolone antibacterial agent moxifloxacin with Cu(II), Fe(III), Mn(II), Ni(II) and VO(II) have been synthesized and characterized by physicochemical and spectroscopic techniques. In these complexes, moxifloxacin acts as a bidentate deprotonated ligand bound to the metal through ketone and carboxylate oxygens. The interactions between the metal complexes and calf thymus DNA have been studied by UV–Vis, circular dichroism and cyclic voltammetry. Fluorescence competitive binding studies with ethidium bromide (EB) demonstrate the ability of the complexes to displace the EB bound to DNA. The cytotoxicities of the complexes have been evaluated on A549 cells by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) method. [Cu(MFL)2(H2O)2] shows the highest anticancer potency. The apoptosis-inducing activity was assessed by acridine orange/ethidium bromide staining assay.

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Acknowledgments

The authors are thankful to University Grants Commission, New Delhi for financial support and are grateful to Head, Dept of Chemistry and Dept of Zoology for providing laboratory facilities. The award of INSPIRE (DST-NEW DELHI) Fellowship to Rinky Singh is also gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, The M.S. University of Baroda, Vadodara, 390002, Gujarat, India

    Rinky Singh & Debjani Chakraborty

  2. Division of Phytotherapeutics and Metabolic Endocrinology, Faculty of Science, The M.S. University of Baroda, Vadodara, 390002, Gujarat, India

    Ravirajsinh N. Jadeja, Menaka C. Thounaojam & Ranjitsinh V. Devkar

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  1. Rinky Singh
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  2. Ravirajsinh N. Jadeja
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  3. Menaka C. Thounaojam
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  4. Ranjitsinh V. Devkar
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  5. Debjani Chakraborty
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Correspondence to Debjani Chakraborty.

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Singh, R., Jadeja, R.N., Thounaojam, M.C. et al. Synthesis, characterization, DNA binding and cytotoxicity studies of moxifloxacinato complexes. Transition Met Chem 37, 541–551 (2012). https://doi.org/10.1007/s11243-012-9620-5

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  • DOI: https://doi.org/10.1007/s11243-012-9620-5

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