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SAMD8 Polyclonal antibody

SAMD8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26815-1-AP

Synonyms

SAMD8, SMSr

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SAMD8 Polyclonal antibody (26815-1-AP)

    WB analysis of mouse heart using 26815-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 26815-1-AP (SAMD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SAMD8 Polyclonal antibody (26815-1-AP)

    IHC staining of human liver cancer using 26815-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SAMD8 Polyclonal antibody (26815-1-AP)

    IHC staining of human liver cancer using 26815-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using SAMD8 Polyclonal antibody (26815-1-AP)

    IHC staining of human heart using 26815-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SAMD8 Polyclonal antibody (26815-1-AP)

    IHC staining of human heart using 26815-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26815-1-AP (SAMD8 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26815-1-AP targets SAMD8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAMD8 fusion protein Ag25310
Full Name sterile alpha motif domain containing 8
Observed Molecular Weight 38 kDa, 45 kDa
GenBank Accession NumberBC146556
Gene Symbol SAMD8
Gene ID (NCBI) 142891
RRIDAB_2880644
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SAMD8 antibody 26815-1-APDownload protocol
IHC protocol for SAMD8 antibody 26815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

J Biol Chem

Sphingomyelin synthase-related protein SMSr is a phosphatidylethanolamine phospholipase C that promotes nonalcoholic fatty liver disease

Authors - Yeun-Po Chiang
View Article